RRC ID 40035
著者 Murmu MS, Martin JR.
タイトル Interaction between cAMP and intracellular Ca(2+)-signaling pathways during odor-perception and adaptation in Drosophila.
ジャーナル Biochim Biophys Acta
Abstract Binding of an odorant to olfactory receptors triggers cascades of second messenger systems in olfactory receptor neurons (ORNs). Biochemical studies indicate that the transduction mechanism at ORNs is mediated by cyclic adenosine monophosphate (cAMP) and/or inositol,1,4,5-triphosphate (InsP3)-signaling pathways in an odorant-dependent manner. However, the interaction between these two second messenger systems during olfactory perception or adaptation processes is much less understood. Here, we used interfering-RNAi to disrupt the level of cAMP alone or in combination with the InsP3-signaling pathway cellular targets, InsP3 receptor (InsP3R) or ryanodine receptor (RyR) in ORNs, and quantify at ORN axon terminals in the antennal lobe, the odor-induced Ca(2+)-response. In-vivo functional bioluminescence Ca(2+)-imaging indicates that a single 5s application of an odor increased Ca(2+)-transients at ORN axon terminals. However, compared to wild-type controls, the magnitude and duration of ORN Ca(2+)-response was significantly diminished in cAMP-defective flies. In a behavioral assay, perception of odorants was defective in flies with a disrupted cAMP level suggesting that the ability of flies to correctly detect an odor depends on cAMP. Simultaneous disruption of cAMP level and InsP3R or RyR further diminished the magnitude and duration of ORN response to odorants and affected the flies' ability to detect an odor. In conclusion, this study provides functional evidence that cAMP and InsP3-signaling pathways act in synergy to mediate odor processing within the ORN axon terminals, which is encoded in the magnitude and duration of ORN response.
巻・号 1863(9)
ページ 2156-74
公開日 2016-9-1
DOI 10.1016/j.bbamcr.2016.05.014
PII S0167-4889(16)30158-6
PMID 27212269
MeSH Adaptation, Physiological* Animals Behavior, Animal Calcium Signaling* Cyclic AMP / metabolism* Drosophila melanogaster / physiology* Gene Knockdown Techniques Inositol 1,4,5-Trisphosphate Receptors / metabolism Intracellular Space / metabolism* Linear Models Models, Biological Neurons / physiology Odorants* Olfactory Perception* Phosphoric Diester Hydrolases / metabolism Receptors, Odorant / metabolism Ryanodine Receptor Calcium Release Channel / metabolism Smell / physiology
IF 3.411
引用数 4
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ 1063R-1 10844R-3 10792R-1 9533R-2