RRC ID |
50844
|
著者 |
Zhu J, Wang HM, Zhang Q, Dong WW, Kong DL, Wang YW, Song JL, Jiang X, Zhao SM, Zhang W, Ruan ZY.
|
タイトル |
Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample.
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ジャーナル |
Antonie Van Leeuwenhoek
|
Abstract |
A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11T, was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11T was found to be able to grow at 4-40 °C (optimum 15 °C), pH 7.5-2.0 (optimum 9.5) and 0-2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11T is closely related to Arenimonas metalli CF5-1T (98.0%), Arenimonas aquaticum NA-09T (97.9%), Arenimonas donghaensis HO3-R19T (95.6%) and Arenimonas aestuarii S2-21T (95.3%). The DNA-DNA hybridization values between the isolate and A. metalli CGMCC 1.10787T, A. aquaticum KACC 14663T, A. donghaensis KACC 11381T were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T m method. The major cellular fatty acids were identified as iso-C15:0 and iso-C16:0. The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11T is concluded to represent a novel species within the genus Arenimonas, for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11T (ACCC 19750T = JCM 30464T).
|
巻・号 |
110(8)
|
ページ |
1027-1034
|
公開日 |
2017-8-1
|
DOI |
10.1007/s10482-017-0876-9
|
PII |
10.1007/s10482-017-0876-9
|
PMID |
28456894
|
MeSH |
Bacteria / genetics
Bacteria / isolation & purification*
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial*
Fatty Acids
Phospholipids
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Soil
Soil Microbiology
|
IF |
2.674
|
引用数 |
3
|
リソース情報 |
一般微生物 |
JCM 30464 |