RRC ID 50844
著者 Zhu J, Wang HM, Zhang Q, Dong WW, Kong DL, Wang YW, Song JL, Jiang X, Zhao SM, Zhang W, Ruan ZY.
タイトル Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11T, was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11T was found to be able to grow at 4-40 °C (optimum 15 °C), pH 7.5-2.0 (optimum 9.5) and 0-2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11T is closely related to Arenimonas metalli CF5-1T (98.0%), Arenimonas aquaticum NA-09T (97.9%), Arenimonas donghaensis HO3-R19T (95.6%) and Arenimonas aestuarii S2-21T (95.3%). The DNA-DNA hybridization values between the isolate and A. metalli CGMCC 1.10787T, A. aquaticum KACC 14663T, A. donghaensis KACC 11381T were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T m method. The major cellular fatty acids were identified as iso-C15:0 and iso-C16:0. The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11T is concluded to represent a novel species within the genus Arenimonas, for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11T (ACCC 19750T = JCM 30464T).
巻・号 110(8)
ページ 1027-1034
公開日 2017-8-1
DOI 10.1007/s10482-017-0876-9
PII 10.1007/s10482-017-0876-9
PMID 28456894
MeSH Bacteria / genetics Bacteria / isolation & purification* Bacterial Typing Techniques Base Composition China DNA, Bacterial* Fatty Acids Phospholipids Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA Soil Soil Microbiology
IF 2.674
引用数 3
リソース情報
一般微生物 JCM 30464