RRC ID 15313
著者 Bryant N, Lloyd J, Sweeney C, Myouga F, Meinke D.
タイトル Identification of nuclear genes encoding chloroplast-localized proteins required for embryo development in Arabidopsis.
ジャーナル Plant Physiol
Abstract We describe here the diversity of chloroplast proteins required for embryo development in Arabidopsis (Arabidopsis thaliana). Interfering with certain chloroplast functions has long been known to result in embryo lethality. What has not been reported before is a comprehensive screen for embryo-defective (emb) mutants altered in chloroplast proteins. From a collection of transposon and T-DNA insertion lines at the RIKEN chloroplast function database (http://rarge.psc.riken.jp/chloroplast/) that initially appeared to lack homozygotes and segregate for defective seeds, we identified 23 additional examples of EMB genes that likely encode chloroplast-localized proteins. Fourteen gene identities were confirmed with allelism tests involving duplicate mutant alleles. We then queried journal publications and the SeedGenes database (www.seedgenes.org) to establish a comprehensive dataset of 381 nuclear genes encoding chloroplast proteins of Arabidopsis associated with embryo-defective (119 genes), plant pigment (121 genes), gametophyte (three genes), and alternate (138 genes) phenotypes. Loci were ranked based on the level of certainty that the gene responsible for the phenotype had been identified and the protein product localized to chloroplasts. Embryo development is frequently arrested when amino acid, vitamin, or nucleotide biosynthesis is disrupted but proceeds when photosynthesis is compromised and when levels of chlorophyll, carotenoids, or terpenoids are reduced. Chloroplast translation is also required for embryo development, with genes encoding chloroplast ribosomal and pentatricopeptide repeat proteins well represented among EMB datasets. The chloroplast accD locus, which is necessary for fatty acid biosynthesis, is essential in Arabidopsis but not in Brassica napus or maize (Zea mays), where duplicated nuclear genes compensate for its absence or loss of function.
巻・号 155(4)
ページ 1678-89
公開日 2011-4-1
DOI 10.1104/pp.110.168120
PII pp.110.168120
PMID 21139083
PMC PMC3091104
MeSH Alleles Arabidopsis / embryology Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Nucleus / genetics* Chloroplasts / metabolism* Gene Expression Regulation, Plant Genetic Complementation Test Mutagenesis, Insertional Mutation Seeds / embryology* Seeds / genetics
IF 6.902
引用数 116
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst18821 pst15134 pst14166 pst14194 pst13173 pst18063 pst00362 pst15471