RRC ID 48747
著者 Kakizaki T, Kitashiba H, Zou Z, Li F, Fukino N, Ohara T, Nishio T, Ishida M.
タイトル A 2-Oxoglutarate-Dependent Dioxygenase Mediates the Biosynthesis of Glucoraphasatin in Radish.
ジャーナル Plant Physiol
Abstract Glucosinolates (GSLs) are secondary metabolites whose degradation products confer intrinsic flavors and aromas to Brassicaceae vegetables. Several structures of GSLs are known in the Brassicaceae, and the biosynthetic pathway and regulatory networks have been elucidated in Arabidopsis (Arabidopsis thaliana). GSLs are precursors of chemical defense substances against herbivorous pests. Specific GSLs can act as feeding blockers or stimulants, depending on the pest species. Natural selection has led to diversity in the GSL composition even within individual species. However, in radish (Raphanus sativus), glucoraphasatin (4-methylthio-3-butenyl glucosinolate) accounts for more than 90% of the total GSLs, and little compositional variation is observed. Because glucoraphasatin is not contained in other members of the Brassicaceae, like Arabidopsis and cabbage (Brassica oleracea), the biosynthetic pathways for glucoraphasatin remain unclear. In this report, we identified and characterized a gene encoding GLUCORAPHASATIN SYNTHASE 1 (GRS1) by genetic mapping using a mutant that genetically lacks glucoraphasatin. Transgenic Arabidopsis, which overexpressed GRS1 cDNA, accumulated glucoraphasatin in the leaves. GRS1 encodes a 2-oxoglutarate-dependent dioxygenase, and it is abundantly expressed in the leaf. To further investigate the biosynthesis and transportation of GSLs in radish, we grafted a grs1 plant onto a wild-type plant. The grafting experiment revealed a leaf-to-root long-distance glucoraphasatin transport system in radish and showed that the composition of GSLs differed among the organs. Based on these observations, we propose a characteristic biosynthesis pathway for glucoraphasatin in radish. Our results should be useful in metabolite engineering for breeding of high-value vegetables.
巻・号 173(3)
ページ 1583-1593
公開日 2017-3-1
DOI 10.1104/pp.16.01814
PII pp.16.01814
PMID 28100450
PMC PMC5338677
MeSH Amino Acid Sequence Arabidopsis / genetics Arabidopsis / metabolism Biological Transport Biosynthetic Pathways / genetics Chromatography, High Pressure Liquid Dioxygenases / classification Dioxygenases / genetics Dioxygenases / metabolism* Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Glucosinolates / analysis Glucosinolates / biosynthesis* Ketoglutaric Acids / metabolism* Metabolic Engineering / methods Mutation Phylogeny Plant Leaves / genetics Plant Leaves / metabolism Plant Roots / genetics Plant Roots / metabolism Plants, Genetically Modified Raphanus / enzymology Raphanus / genetics Raphanus / metabolism* Reverse Transcriptase Polymerase Chain Reaction Seedlings / genetics Seedlings / metabolism Seedlings / physiology Sequence Homology, Amino Acid
IF 6.902
引用数 13
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 psw00001