RRC ID 35568
著者 Arora S, Bhamidimarri SP, Bhattacharyya M, Govindan A, Weber MH, Vishveshwara S, Varshney U.
タイトル Distinctive contributions of the ribosomal P-site elements m2G966, m5C967 and the C-terminal tail of the S9 protein in the fidelity of initiation of translation in Escherichia coli.
ジャーナル Nucleic Acids Res
Abstract The accuracy of pairing of the anticodon of the initiator tRNA (tRNA(fMet)) and the initiation codon of an mRNA, in the ribosomal P-site, is crucial for determining the translational reading frame. However, a direct role of any ribosomal element(s) in scrutinizing this pairing is unknown. The P-site elements, m(2)G966 (methylated by RsmD), m(5)C967 (methylated by RsmB) and the C-terminal tail of the protein S9 lie in the vicinity of tRNA(fMet). We investigated the role of these elements in initiation from various codons, namely, AUG, GUG, UUG, CUG, AUA, AUU, AUC and ACG with tRNA(fMet(CAU) (tRNA(fMet) with CAU anticodon); CAC and CAU with tRNA(fMet(GUG); UAG with tRNA(fMet(CAU) ; UAC with tRNA(fMet(GUG) ; and AUC with tRNA(fMet(GUG) using in vivo and computational methods. Although RsmB deficiency did not impact initiation from most codons, RsmD deficiency increased initiation from AUA, CAC and CAU (2- to 3.6-fold). Deletion of the S9 C-terminal tail resulted in poorer initiation from UUG, GUG and CUG, but in increased initiation from CAC, CAU and UAC codons (up to 4-fold). Also, the S9 tail suppressed initiation with tRNA(fMet(CAU) lacking the 3GC base pairs in the anticodon stem. These observations suggest distinctive roles of 966/967 methylations and the S9 tail in initiation.
巻・号 41(9)
ページ 4963-75
公開日 2013-5-1
DOI 10.1093/nar/gkt175
PII gkt175
PMID 23530111
PMC PMC3643588
MeSH Anticodon Codon Escherichia coli / genetics* Escherichia coli / metabolism Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Methylation Molecular Dynamics Simulation Mutation Peptide Chain Initiation, Translational* RNA, Messenger / chemistry RNA, Ribosomal, 16S / chemistry* RNA, Ribosomal, 16S / metabolism RNA, Transfer, Met / chemistry* RNA, Transfer, Met / genetics RNA, Transfer, Met / metabolism Ribosomal Protein S9 Ribosomal Proteins / chemistry* Ribosomal Proteins / genetics Ribosomes / chemistry* Sequence Deletion
IF 11.502
引用数 17
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113)