RRC ID |
50027
|
著者 |
Mukai H, Takahashi M, Watanabe Y.
|
タイトル |
Potential usefulness of Brevibacillus for bacterial cancer therapy: intratumoral provision of tumor necrosis factor-α and anticancer effects.
|
ジャーナル |
Cancer Gene Ther
|
Abstract |
Bacterial cancer therapy, wherein bacteria are used as a gene expression system for the exogenous protein of interest in the body, has started becoming a focus area of research; therefore, studying potential bacterial species for use is extremely important. Here, we investigated the use of Brevibacillus choshinensis as an effective and safe provider of anticancer proteins in the body, using a transformant expressing murine tumor necrosis factor-α (mTNF-α). The transformant sustainably provided mTNF-α in tumors in mice for a few hours post-injection. The growth of TNF-α-sensitive tumors was inhibited even by the control transformant, which did not provide mTNF-α; intratumoral mTNF-α provision by Brevibacillus choshinensis had additive effects on tumor growth inhibition. In contrast, intratumorally injected recombinant mTNF-α did not inhibit tumor growth because of rapid elimination from the tumor. Blood biochemical and histochemical analyses showed that intravenous injection of the transformant that did not provide mTNF-α did not lead to tissue injury and dysfunction or infiltration of inflammatory cells over 1 week. Considering the findings, this approach is expected to have a high degree of usability as a delivery system for protein pharmaceuticals, especially from the viewpoints of loading capacity and cost effectiveness.
|
巻・号 |
25(3-4)
|
ページ |
47-57
|
公開日 |
2018-5-1
|
DOI |
10.1038/s41417-017-0009-7
|
PII |
10.1038/s41417-017-0009-7
|
PMID |
29255215
|
MeSH |
Animals
Brevibacillus* / genetics
Brevibacillus* / immunology
Cell Line, Tumor
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Mice, SCID
Neoplasms, Experimental* / genetics
Neoplasms, Experimental* / immunology
Neoplasms, Experimental* / therapy
Tumor Necrosis Factor-alpha* / genetics
Tumor Necrosis Factor-alpha* / immunology
|
IF |
4.534
|
引用数 |
1
|
リソース情報 |
遺伝子材料 |
pBluescript SKII+mTNF alpha (RDB01478). |
ヒト・動物細胞 |
L929(RCB1422) |