RRC ID 33161
著者 Hartman TR, Ventresca EM, Hopkins A, Zinshteyn D, Singh T, O'Brien JA, Neubert BC, Hartman MG, Schofield HK, Stavrides KP, Talbot DE, Riggs DJ, Pritchard C, O'Reilly AM.
タイトル Novel tools for genetic manipulation of follicle stem cells in the Drosophila ovary reveal an integrin-dependent transition from quiescence to proliferation.
ジャーナル Genetics
Abstract In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the Drosophila ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.
巻・号 199(4)
ページ 935-57
公開日 2015-4-1
DOI 10.1534/genetics.114.173617
PII genetics.114.173617
PMID 25680813
PMC PMC4391569
MeSH Animals Cell Proliferation Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Female Gene Expression Profiling / methods* Genome, Insect* Integrins / genetics Integrins / metabolism* Male Ovarian Follicle / cytology* Stem Cells / cytology Stem Cells / metabolism* Stem Cells / physiology
IF 4.015
引用数 9
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ y[*] P{GawB}boi[NP4065] w[*]/FM7c (DGRC#104588) w[*] P{GawB}NP4124/FM7c (DGRC#113397) P{w[+mW.hs]=GawB}sgg[NP0082] w[*]/FM7c (DGRC#112033) y[*] P{w[+mW.hs]=GawB}sgg[NP2253] w[*]/FM7c (DGRC#104140) y[*] P{w[+mW.hs]=GawB}sgg[NP4101] w[*]/FM7c (DGRC#104603) y[*] P{w[+mW.hs]=GawB}sgg[NP7167] w[*]/FM7c (DGRC#114115) P{w[+mW.hs]=GawB}sgg[NP7270] w[*]/FM7c (DGRC#114191) y[*] w[*] P{w[+mW.hs]=GawB}sli[NP1625]/CyO P{w[?]=UAS-lacZ.UW14}UW14 (DGRC#104056) and w[*] P{w[+mW.hs]=GawB}sli[NP2755]/CyO (DGRC#113037)