RRC ID 45910
著者 Dejima K, Kang S, Mitani S, Cosman PC, Chisholm AD.
タイトル Syndecan defines precise spindle orientation by modulating Wnt signaling in C. elegans.
ジャーナル Development
Abstract Wnt signals orient mitotic spindles in development, but it remains unclear how Wnt signaling is spatially controlled to achieve precise spindle orientation. Here, we show that C. elegans syndecan (SDN-1) is required for precise orientation of a mitotic spindle in response to a Wnt cue. We find that SDN-1 is the predominant heparan sulfate (HS) proteoglycan in the early C. elegans embryo, and that loss of HS biosynthesis or of the SDN-1 core protein results in misorientation of the spindle of the ABar blastomere. The ABar and EMS spindles both reorient in response to Wnt signals, but only ABar spindle reorientation is dependent on a new cell contact and on HS and SDN-1. SDN-1 transiently accumulates on the ABar surface as it contacts C, and is required for local concentration of Dishevelled (MIG-5) in the ABar cortex adjacent to C. These findings establish a new role for syndecan in Wnt-dependent spindle orientation.
巻・号 141(22)
ページ 4354-65
公開日 2014-11-1
DOI 10.1242/dev.113266
PII dev.113266
PMID 25344071
PMC PMC6514391
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / metabolism Fluorescent Antibody Technique Microscopy, Confocal RNA Interference Spindle Apparatus / physiology* Syndecan-1 / metabolism* Wnt Signaling Pathway / physiology*
IF 5.611
引用数 15
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm2051 tm516 tm710