RRC ID 41006
著者 Chen N, Yu XJ, Yang JS, Wang ET, Li BZ, Yuan HL.
タイトル Novosphingobium tardum sp. nov., isolated from sediment of a freshwater lake.
ジャーナル Antonie Van Leeuwenhoek
Abstract A Gram-negative, non-motive, aerobic and non-spore-forming strain 16-28-2(T) isolated from freshwater sediment of Taihu Lake was characterized by using a polyphasic approach. The optimum growth conditions were found to be as follows: 28 °C, pH 6.5 and 0-0.5 % NaCl in YG liquid medium. The major fatty acids were identified to be summed feature 3 (consisting of C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (consisting of C18:1 ω7c and/or C18:1 ω6c), C14:0 2-OH, C17:1 ω6c, C16:0 and C18:1 ω7c 11-methyl (>5 %). Strain 16-28-2(T) was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid as the major polar lipids; and ubiquinone 10 (Q-10) as the major respiratory quinone. DNA G+C content of strain 16-28-2(T) was 63.5 mol % (Tm). A phylogenetic study of 16S rRNA gene indicated that strain 16-28-2(T) is a member of the genus Novosphingobium, with the highest 16S rRNA gene sequence similarity of 96.3 % with Novosphingobium lentum MT1(T) and below 96 % with the other Novosphingobium species. On the basis of the phylogenetic, phenotypic analyses and biochemical characterization, we suggest that strain 16-28-2(T) is a novel species in the genus Novosphingobium, for which the name Novosphingobium tardum sp. nov. is proposed. The type strain of N. tardum is 16-28-2(T) (=CGMCC 1.12989(T) =NBRC 110956(T)).
巻・号 108(1)
ページ 51-7
公開日 2015-7-1
DOI 10.1007/s10482-015-0463-x
PMID 25912732
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Fresh Water Geologic Sediments / microbiology* Glycolipids / analysis Hydrogen-Ion Concentration Lakes Molecular Sequence Data Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Sphingomonadaceae / classification* Sphingomonadaceae / genetics Sphingomonadaceae / isolation & purification* Sphingomonadaceae / physiology Temperature
IF 2.674
引用数 5
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19923