RRC ID 41076
著者 Kang JP, Nguyen NL, Kim YJ, Hoang VA, Bae KS, Yang DC.
タイトル Paralcaligenes ginsengisoli sp. nov., isolated from ginseng cultivated soil.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel bacterial strain DCY104(T) isolated from soil of a ginseng field in Yeoncheon County, Republic of Korea is described in this study. Cells were short rod-shaped, motile by mean of one polar flagellum, strictly aerobic, Gramreaction negative, oxidase and catalase-positive. 16S rRNA gene sequence analysis showed that strain DCY104(T) shared highest similarity 98.2 % to Paralcaligenes ureilyticus GR24-5(T), and from 97.7 to 97.1 % with other type strains belong to the genera Candidimonas, Pusillimonas and Parapusillimonas Otherwise, phylogenetic trees analyses indicated that strain DCY104(T) belongs to a single group with P. ureilyticus GR24-5(T) that was distinct to other genera. The major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The major cellular fatty acids consisted of C16:0, cyclo-C17:0, and summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c). The predominant polyamine was putrescine. The ubiquinone was Q-8. The genomic DNA G+C content was 55.9 mol%. These data in combination with the presence of one polar flagellum and positive activity of urease confirmed the placement of strain DCY104(T) in the genus Paralcaligenes. DNA-DNA relatedness between strain DCY104(T) and P. ureilyticus KACC 13888(T) was 40 %. The differences in the profiles of polar lipids, fatty acids and phenotypic characteristics in combination with DNA-DNA relatedness delineated strain DCY104(T) and P. ureilyticus KACC 13888(T). In summary, taxonomic analyses in this study demonstrated that strain DCY104(T) represents a novel species within the genus Paralcaligenes, for which we propose the name Paralcaligenes ginsengisoli. The type strain is DCY104(T) (= KCTC 42406(T) = JCM 30746(T)).
巻・号 108(3)
ページ 619-26
公開日 2015-9-1
DOI 10.1007/s10482-015-0517-0
PMID 26122887
MeSH Aerobiosis Alcaligenaceae / classification* Alcaligenaceae / genetics Alcaligenaceae / isolation & purification* Alcaligenaceae / physiology Bacterial Typing Techniques Base Composition Catalase / analysis Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Korea Locomotion Molecular Sequence Data Nucleic Acid Hybridization Oxidoreductases / analysis Panax / growth & development* Phospholipids / analysis Phylogeny Putrescine / analysis Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology*
IF 2.674
引用数 6
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30746