RRC ID 36992
著者 Shigeta T, Zaizen Y, Sugimoto Y, Nakamura Y, Matsuo T, Okamoto S.
タイトル Heat shock protein 90 acts in brassinosteroid signaling through interaction with BES1/BZR1 transcription factor.
ジャーナル J Plant Physiol
Abstract Brassinosteroids (BRs), a class of phytohormones, control various physiological and developmental processes in plants. Two highly homologous transcription factors, brassinosteroid insensitive 1-EMS-SUPRESSOR 1 (BES1) and brassinazole resistant 1 (BZR1), act downstream of BR signaling to control several thousands of putative target genes. We reported previously that BES1 forms a complex with a molecular chaperone: heat shock protein 90 (HSP90). This study demonstrates that the amino-terminal and central parts of BES1 are responsible for its physical interaction with HSP90.3 in vitro. Additionally, we present evidence that BZR1 is a novel HSP90 partner aside from two BR signaling components previously identified as its clients: BES1 and brassinosteroid insensitive 2 (BIN2). Furthermore, geldanamycin, an inhibitor of ATPase activity in HSP90, caused BES1 hyperphosphorylation and disrupted the expression of BR-responsive genes. Considered together, our results imply that HSP90 takes a part in BR-mediated gene expression through complex formation with two major transcription factors.
巻・号 178
ページ 69-73
公開日 2015-4-15
DOI 10.1016/j.jplph.2015.02.003
PII S0176-1617(15)00041-3
PMID 25778412
MeSH Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Benzoquinones / pharmacology Brassinosteroids / metabolism* DNA-Binding Proteins Gene Expression Regulation / drug effects* HSP90 Heat-Shock Proteins / genetics* HSP90 Heat-Shock Proteins / metabolism Lactams, Macrocyclic / pharmacology Nuclear Proteins / genetics* Nuclear Proteins / metabolism Phosphorylation Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism
IF 3.013
引用数 15
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008