RRC ID |
52662
|
著者 |
Okubo Y, Kasamatsu A, Yamatoji M, Fushimi K, Ishigami T, Shimizu T, Kasama H, Shiiba M, Tanzawa H, Uzawa K.
|
タイトル |
Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.
|
ジャーナル |
Exp Cell Res
|
Abstract |
Diacylglycerol lipase alpha (DAGLA), which catalyzes the hydrolysis of diacylglycerol to 2-arachidonoylglycerol and free fatty acid, is required for axonal growth during the brain development and for retrograde synaptic signaling at mature synapses. So far, no information was found regarding the possible role of DAGLA in human tumorigenesis. Thus, the current study sought to clarify the contribution of DAGLA in oral squamous cell carcinomas (OSCCs) and assess the clinical possibilities for OSCC treatment. Using real-time quantitative reverse transcription-polymerase chain reaction, immunoblotting, and immunohistochemistry, we found a significant up-regulation of DAGLA in OSCCs compared with normal cells and tissues both at mRNA and protein expression levels. Knockdown models in OSCC-derived cell lines for DAGLA (siDAGLA) and treatment with a lipase inhibitor (orlistat) showed several depressed cellular functions, including cellular proliferation and migratory activities through cell-cycle arrest at G1 phase. Furthermore, we found that DAGLA-positive OSCC samples were correlated highly with the primary tumoral size. We concluded that DAGLA may be a key determinant in tumoral progression and might be a therapeutic target for OSCCs.
|
巻・号 |
367(1)
|
ページ |
112-118
|
公開日 |
2018-6-1
|
DOI |
10.1016/j.yexcr.2018.03.041
|
PII |
S0014-4827(18)30202-7
|
PMID |
29614312
|
MeSH |
Animals
Carcinogenesis / genetics*
Carcinoma, Squamous Cell / enzymology
Carcinoma, Squamous Cell / pathology*
Cell Cycle / drug effects*
Cell Line, Tumor
Cell Proliferation / drug effects
Female
Humans
Lipoprotein Lipase / antagonists & inhibitors
Lipoprotein Lipase / genetics
Lipoprotein Lipase / metabolism*
Mice
Mice, Nude
Mouth Neoplasms / enzymology
Mouth Neoplasms / pathology*
Orlistat / pharmacology
Primary Cell Culture
Xenograft Model Antitumor Assays
|
IF |
3.383
|
引用数 |
4
|
リソース情報 |
ヒト・動物細胞 |
HSC-2(RCB1945)
HSC-3(RCB1975)
HSC-4(RCB1902)
Sa3(RCB0980)
Ca9-22(RCB1976)
SAS(RCB1974)
Ho-1-u-1(RCB2102) |