RRC ID 36872
著者 Suzuki Y, Nakagawa K, Kato S, Tatewaki N, Mizuochi S, Ito J, Eitsuka T, Nishida H, Miyazawa T.
タイトル Metabolism and cytotoxic effects of phosphatidylcholine hydroperoxide in human hepatoma HepG2 cells.
ジャーナル Biochem Biophys Res Commun
Abstract In this study, we investigated cellular uptake and metabolism of phosphatidylcholine hydroperoxide (PCOOH) in human hepatoma HepG2 cells by high performance liquid chromatography-tandem mass spectrometry, and then evaluated whether PCOOH or its metabolites cause pathophysiological effects such as cytotoxicity and apoptosis. Although we found that most PCOOH was reduced to PC hydroxide in HepG2 cells, the remaining PCOOH caused cytotoxic effects that may be mediated through an unusual apoptosis pathway. These results will enhance our fundamental understanding of how PCOOH, which is present in oxidized low density lipoproteins, is involved in the development of atherosclerosis.
巻・号 458(4)
ページ 920-7
公開日 2015-3-20
DOI 10.1016/j.bbrc.2015.02.063
PII S0006-291X(15)00291-0
PMID 25704087
MeSH Apoptosis* Cell Cycle Hep G2 Cells / cytology* Hep G2 Cells / metabolism Hep G2 Cells / pathology Humans Membrane Potential, Mitochondrial Phosphatidylcholines / metabolism* Phosphatidylcholines / toxicity
IF 2.985
引用数 3
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 Hep G2(RCB1886)