RRC ID |
49577
|
著者 |
Ren B, Tan HL, Nguyen TTT, Sayed AMM, Li Y, Mok YK, Yang H, Chen ES.
|
タイトル |
Regulation of transcriptional silencing and chromodomain protein localization at centromeric heterochromatin by histone H3 tyrosine 41 phosphorylation in fission yeast.
|
ジャーナル |
Nucleic Acids Res
|
Abstract |
Heterochromatin silencing is critical for genomic integrity and cell survival. It is orchestrated by chromodomain (CD)-containing proteins that bind to methylated histone H3 lysine 9 (H3K9me), a hallmark of heterochromatin. Here, we show that phosphorylation of tyrosine 41 (H3Y41p)-a novel histone H3 modification-participates in the regulation of heterochromatin in fission yeast. We show that a loss-of-function mutant of H3Y41 can suppress heterochromatin de-silencing in the centromere and subtelomere repeat regions, suggesting a de-silencing role for H3Y41p on heterochromatin. Furthermore, we show both in vitro and in vivo that H3Y41p differentially regulates two CD-containing proteins without the change in the level of H3K9 methylation: it promotes the binding of Chp1 to histone H3 and the exclusion of Swi6. H3Y41p is preferentially enriched on centromeric heterochromatin during M- to early S phase, which coincides with the localization switch of Swi6/Chp1. The loss-of-function H3Y41 mutant could suppress the hypersensitivity of the RNAi mutants towards hydroxyurea (HU), which arrests replication in S phase. Overall, we describe H3Y41p as a novel histone modification that differentially regulates heterochromatin silencing in fission yeast via the binding of CD-containing proteins.
|
巻・号 |
46(1)
|
ページ |
189-202
|
公開日 |
2018-1-9
|
DOI |
10.1093/nar/gkx1010
|
PII |
4612962
|
PMID |
29136238
|
PMC |
PMC5758876
|
MeSH |
Cell Cycle Checkpoints / genetics
Centromere / genetics*
Centromere / metabolism
Heterochromatin / genetics*
Heterochromatin / metabolism
Histones / genetics*
Histones / metabolism
Loss of Function Mutation
Methylation
Phosphorylation
RNA Interference*
S Phase / genetics
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
Tyrosine / genetics
Tyrosine / metabolism
|
IF |
11.502
|
引用数 |
2
|
リソース情報 |
酵母 |
FY20405
FY20348
FY21303
FY21304
FY20867
FY8496
FY8495
FY8734
FY8450
FY20045
FY20044
FY20043
FY20038
FY20042
FY20041
FY20040
FY20039
FY20866 |