RRC ID 39989
著者 Chow RW, Almeida AD, Randlett O, Norden C, Harris WA.
タイトル Inhibitory neuron migration and IPL formation in the developing zebrafish retina.
ジャーナル Development
Abstract The mature vertebrate retina is a highly ordered neuronal network of cell bodies and synaptic neuropils arranged in distinct layers. Little, however, is known about the emergence of this spatial arrangement. Here, we investigate how the three main types of retinal inhibitory neuron (RIN)--horizontal cells (HCs), inner nuclear layer amacrine cells (iACs) and displaced amacrine cells (dACs)--reach their specific laminar positions during development. Using in vivo time-lapse imaging of zebrafish retinas, we show that RINs undergo distinct phases of migration. The first phase, common to all RINs, is bipolar migration directed towards the apicobasal centre of the retina. All RINs then transition to a less directionally persistent multipolar phase of migration. Finally, HCs, iACs and dACs each undergo cell type-specific migration. In contrast to current hypotheses, we find that most dACs send processes into the forming inner plexiform layer (IPL) before migrating through it and inverting their polarity. By imaging and quantifying the dynamics of HCs, iACs and dACs from birth to final position, this study thus provides evidence for distinct and new migration patterns during retinal lamination and insights into the initiation of IPL formation.
巻・号 142(15)
ページ 2665-77
公開日 2015-8-1
DOI 10.1242/dev.122473
PII dev.122473
PMID 26116662
PMC PMC4529032
MeSH Animals Animals, Genetically Modified Cell Movement / physiology* Image Processing, Computer-Assisted Kinetics Microscopy, Fluorescence Neurons / cytology Neurons / physiology* Retina / embryology* Time-Lapse Imaging Zebrafish / embryology*
IF 5.611
引用数 13
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ゼブラフィッシュ Tg(vsx1:GFP)