論文 - 詳細
RRC ID | 31980 |
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著者 | Kim SN, Jeibmann A, Halama K, Witte HT, Wälte M, Matzat T, Schillers H, Faber C, Senner V, Paulus W, Klämbt C. |
タイトル | ECM stiffness regulates glial migration in Drosophila and mammalian glioma models. |
ジャーナル | Development |
Abstract |
Cell migration is an important feature of glial cells. Here, we used the Drosophila eye disc to decipher the molecular network controlling glial migration. We stimulated glial motility by pan-glial PDGF receptor (PVR) activation and identified several genes acting downstream of PVR. Drosophila lox is a non-essential gene encoding a secreted protein that stiffens the extracellular matrix (ECM). Glial-specific knockdown of Integrin results in ECM softening. Moreover, we show that lox expression is regulated by Integrin signaling and vice versa, suggesting that a positive-feedback loop ensures a rigid ECM in the vicinity of migrating cells. The general implication of this model was tested in a mammalian glioma model, where a Lox-specific inhibitor unraveled a clear impact of ECM rigidity in glioma cell migration. |
巻・号 | 141(16) |
ページ | 3233-42 |
公開日 | 2014-8-1 |
DOI | 10.1242/dev.106039 |
PII | dev.106039 |
PMID | 25063458 |
MeSH | Animals Animals, Genetically Modified Base Sequence Cell Line, Tumor Cell Movement Compound Eye, Arthropod / embryology* Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Extracellular Matrix / metabolism Extracellular Matrix / physiology* Female Gene Expression Regulation, Developmental Glioblastoma / metabolism Humans Integrins / metabolism Mice Mice, Nude Molecular Sequence Data Neoplasm Transplantation Neuroglia / cytology* Protein-Lysine 6-Oxidase / genetics Protein-Lysine 6-Oxidase / physiology* Signal Transduction |
IF | 5.611 |
引用数 | 38 |
WOS 分野 | DEVELOPMENTAL BIOLOGY |
リソース情報 | |
ショウジョウバエ | 11335R-3 |