RRC ID 31980
著者 Kim SN, Jeibmann A, Halama K, Witte HT, Wälte M, Matzat T, Schillers H, Faber C, Senner V, Paulus W, Klämbt C.
タイトル ECM stiffness regulates glial migration in Drosophila and mammalian glioma models.
ジャーナル Development
Abstract Cell migration is an important feature of glial cells. Here, we used the Drosophila eye disc to decipher the molecular network controlling glial migration. We stimulated glial motility by pan-glial PDGF receptor (PVR) activation and identified several genes acting downstream of PVR. Drosophila lox is a non-essential gene encoding a secreted protein that stiffens the extracellular matrix (ECM). Glial-specific knockdown of Integrin results in ECM softening. Moreover, we show that lox expression is regulated by Integrin signaling and vice versa, suggesting that a positive-feedback loop ensures a rigid ECM in the vicinity of migrating cells. The general implication of this model was tested in a mammalian glioma model, where a Lox-specific inhibitor unraveled a clear impact of ECM rigidity in glioma cell migration.
巻・号 141(16)
ページ 3233-42
公開日 2014-8-1
DOI 10.1242/dev.106039
PII dev.106039
PMID 25063458
MeSH Animals Animals, Genetically Modified Base Sequence Cell Line, Tumor Cell Movement Compound Eye, Arthropod / embryology* Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Extracellular Matrix / metabolism Extracellular Matrix / physiology* Female Gene Expression Regulation, Developmental Glioblastoma / metabolism Humans Integrins / metabolism Mice Mice, Nude Molecular Sequence Data Neoplasm Transplantation Neuroglia / cytology* Protein-Lysine 6-Oxidase / genetics Protein-Lysine 6-Oxidase / physiology* Signal Transduction
IF 5.611
引用数 38
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 11335R-3