| RRC ID |
36738
|
| 著者 |
Francoz E, Ranocha P, Pernot C, Le Ru A, Pacquit V, Dunand C, Burlat V.
|
| タイトル |
Complementarity of medium-throughput in situ RNA hybridization and tissue-specific transcriptomics: case study of Arabidopsis seed development kinetics.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The rationale of this study is to compare and integrate two heterologous datasets intended to unravel the spatiotemporal specificities of gene expression in a rapidly growing and complex organ. We implemented medium-throughput RNA in situ hybridization (ISH) for 39 genes mainly corresponding to cell wall proteins for which we have particular interest, selected (i) on their sequence identity (24 class III peroxidase multigenic family members and 15 additional genes used as positive controls) and (ii) on their expression levels in a publicly available Arabidopsis thaliana seed tissue-specific transcriptomics study. The specificity of the hybridization signals was carefully studied, and ISH results obtained for the 39 selected genes were systematically compared with tissue-specific transcriptomics for 5 seed developmental stages. Integration of results illustrates the complementarity of both datasets. The tissue-specific transcriptomics provides high-throughput possibilities whereas ISH provides high spatial resolution. Moreover, depending on the tissues and the developmental stages considered, one or the other technique appears more sensitive than the other. For each tissue/developmental stage, we finally determined tissue-specific transcriptomic threshold values compatible with the spatiotemporally-specific detection limits of ISH for lists of hundreds to tens-of-thousands of genes.
|
| 巻・号 |
6
|
| ページ |
24644
|
| 公開日 |
2016-4-20
|
| DOI |
10.1038/srep24644
|
| PII |
srep24644
|
| PMID |
27095274
|
| PMC |
PMC4837347
|
| MeSH |
Arabidopsis / genetics*
Databases, Nucleic Acid
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
In Situ Hybridization* / methods
Organ Specificity / genetics
Seeds / genetics*
Transcriptome*
|
| IF |
3.998
|
| 引用数 |
6
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
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