RRC ID 33638
著者 Huang MS, Wang TK, Liu YW, Li YT, Chi TH, Chou CW, Hsieh M.
タイトル Roles of carbonic anhydrase 8 in neuronal cells and zebrafish.
ジャーナル Biochim Biophys Acta
Abstract BACKGROUND:Carbonic anhydrase 8 (CA8) is an isozyme of α-carbonic anhydrases (CAs). Previous studies showed that CA8 can be detected in human adult brain, with more intense expression in the cerebellum. Single mutations in CA8 were reported to cause novel syndromes like ataxia, mild mental retardation or the predisposition to quadrupedal gait.
METHODS:In the present study, we examine the functions of CA8 in neuronal cell lines, mouse cerebellar granule neurons and zebrafish.
RESULTS AND CONCLUSIONS:We demonstrated that overexpression of CA8 in neuronal cells significantly decreased cell death under staurosporine treatment. Moreover, CA8 overexpression significantly increased cell migration and invasion ability in neuronal cells and in mouse cerebellar granule neurons, implicating that CA8 may be involved in neuron motility and oncogenesis. By using zebrafish as an animal model, motor reflection of 3dpf zebrafish embryos was significantly affected after the down-regulation of CA8 through ca8 morpholino.
CONCLUSIONS:We concluded that CA8 overexpression desensitizes neuronal cells to STS induced apoptotic stress and increases cell migration and invasion ability in neuronal cells. In addition, down-regulated CA8 decreases neuron mobility in neuronal cells and leads to abnormal calcium release in cerebellar granule neurons. Knockdown of the ca8 gene results in an abnormal movement pattern in zebrafish.
GENERAL SIGNIFICANCE:Our findings provide evidence to support that the impaired protective function of CA8 contributes to human neuropathology, and to suggest that zebrafish can be used as an animal model to study the biological functions of human CA8 in vivo.
巻・号 1840(9)
ページ 2829-42
公開日 2014-9-1
DOI 10.1016/j.bbagen.2014.04.017
PII S0304-4165(14)00150-0
PMID 24794067
MeSH Animals Biomarkers, Tumor / biosynthesis* Biomarkers, Tumor / genetics Cell Line Cerebellum / enzymology* Cerebellum / pathology Disease Models, Animal Gene Knockdown Techniques Humans Mice Mice, Inbred ICR Nerve Tissue Proteins / biosynthesis* Nerve Tissue Proteins / genetics Nervous System Diseases / enzymology* Nervous System Diseases / genetics Nervous System Diseases / pathology Neurons / enzymology* Neurons / pathology Zebrafish Zebrafish Proteins / biosynthesis* Zebrafish Proteins / genetics
IF 3.411
引用数 8
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ゼブラフィッシュ isl1:GFP/rw0