RRC ID 45661
著者 Yang J, Fong HT, Xie Z, Tan JW, Inoue T.
タイトル Direct and positive regulation of Caenorhabditis elegans bed-3 by PRDM1/BLIMP1 ortholog BLMP-1.
ジャーナル Biochim Biophys Acta
Abstract Caenorhabditis elegans BLMP-1 is the homolog of mammalian PRDM1/BLIMP1 transcriptional repressor and a component of the heterochronic pathway regulating developmental timing. We found that BLMP-1 positively and directly regulates the bed-3 gene required for vulval cell division and molting. blmp-1 mutation or RNAi reduces bed-3 reporter expression and causes phenotype similar to bed-3 mutations. We mapped an enhancer element responsible for bed-3 expression in the vulva and the hypodermis to a 200 bp region in the third intron. Using EMSA, we identified BLMP-1 binding sites within this region. Mutating these sites abolished both in vitro BLMP-1 binding and in vivo enhancer activity. Thus, BLMP-1 directly and positively regulates bed-3 transcription, connecting the heterochronic pathway to regulation of vulval cell division and molting. Recently, C. elegans blmp-1 was found to act as a transcriptional repressor. Thus, C. elegans BLMP-1 acts as a transcriptional activator or a repressor depending on the context.
巻・号 1849(9)
ページ 1229-36
公開日 2015-9-1
DOI 10.1016/j.bbagrm.2015.07.012
PII S1874-9399(15)00164-9
PMID 26234645
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Enhancer Elements, Genetic Gene Expression Regulation / physiology* Introns Transcription Factors / genetics* Transgenes
IF 3.411
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
線虫 NA