RRC ID 37882
著者 Matsuura K, Seta H, Haraguchi Y, Alsayegh K, Sekine H, Shimizu T, Hagiwara N, Yamazaki K, Okano T.
タイトル TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues.
ジャーナル Sci Rep
Abstract The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, we show for the first time that TRPV-1 activation through transient culture at 42 °C in combination with agonists is a simple and useful strategy to eliminate iPS cells from bioengineered cardiac cell sheet tissues. When human iPS cells were cultured at 42 °C, almost all cells disappeared by 48 hours through apoptosis. However, iPS cell-derived cardiomyocytes and fibroblasts maintained transcriptional and protein expression levels, and cardiac cell sheets were fabricated after reducing the temperature. TRPV-1 expression in iPS cells was upregulated at 42 °C, and iPS cell death at 42 °C was TRPV-1-dependent. Furthermore, TRPV-1 activation through thermal or agonist treatment eliminated iPS cells in cardiac tissues for a final concentration of 0.4% iPS cell contamination. These findings suggest that the difference in tolerance to TRPV-1 activation between iPS cells and iPS cell-derived cardiac cells could be exploited to eliminate remaining iPS cells in bioengineered cell sheet tissues, which will further reduce the risk of tumour formation.
巻・号 6
ページ 21747
公開日 2016-2-18
DOI 10.1038/srep21747
PII srep21747
PMID 26888607
PMC PMC4757885
MeSH Cell Differentiation Cells, Cultured Coculture Techniques Fibroblasts / cytology Fibroblasts / metabolism Hot Temperature Humans Induced Pluripotent Stem Cells / cytology* Myocytes, Cardiac / cytology* Myocytes, Cardiac / metabolism TRPV Cation Channels / agonists TRPV Cation Channels / genetics* TRPV Cation Channels / metabolism* Tissue Engineering / methods*
IF 3.998
引用数 14
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 201B7(HPS0063) 253G1(HPS0002)