RRC ID 37460
著者 Shiraishi H.
タイトル Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis.
ジャーナル Biosci Biotechnol Biochem
Abstract Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately -1 °C min(-1) in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately -1 °C min(-1) in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.
巻・号 80(10)
ページ 2051-7
公開日 2016-10-1
DOI 10.1080/09168451.2016.1189320
PMID 27240586
MeSH Cell Proliferation Cell Survival Cryopreservation / methods* Species Specificity Spirulina / cytology*
IF 1.516
引用数 5
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY
リソース情報
藻類 NIES-39 NIES-46 NIES-2308