RRC ID 11405
著者 Shahriari M, Keshavaiah C, Scheuring D, Sabovljevic A, Pimpl P, Häusler RE, Hülskamp M, Schellmann S.
タイトル The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of Arabidopsis thaliana.
ジャーナル Plant J
Abstract The vacuole is the most prominent organelle of plant cells. Despite its importance for many physiological and developmental aspects of plant life, little is known about its biogenesis and maintenance. Here we show that Arabidopsis plants expressing a dominant-negative version of the AAA (ATPase associated with various cellular activities) ATPase AtSKD1 (SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) under the control of the trichome-specific GLABRA2 (GL2) promoter exhibit normal vacuolar development in early stages of trichome development. Shortly after its formation, however, the large central vacuole is fragmented and finally disappears completely. Secretion assays with amylase fused to the vacuolar sorting signal of Sporamin show that dominant-negative AtSKD1 inhibits vacuolar trafficking of the reporter that is instead secreted. In addition, trichomes expressing dominant-negative AtSKD1 frequently contain multiple nuclei. Our results suggest that AtSKD1 contributes to vacuolar protein trafficking and thereby to the maintenance of the large central vacuole of plant cells, and might play a role in cell-cycle regulation.
巻・号 64(1)
ページ 71-85
公開日 2010-10-1
DOI 10.1111/j.1365-313X.2010.04310.x
PII TPJ4310
PMID 20663085
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Nucleus / metabolism DNA, Plant / genetics Endosomes / metabolism Gene Expression Regulation, Plant Mutagenesis, Site-Directed Promoter Regions, Genetic Vacuoles / metabolism*
IF 6.141
引用数 42
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda11165 pda02568 pda02482 pda01520 pda11379 pda04291 pda01247 pda07337 pda02603