RRC ID 39786
著者 Inoue H, Ogonuki N, Hirose M, Hatanaka Y, Matoba S, Chuma S, Kobayashi K, Wakana S, Noguchi J, Inoue K, Tanemura K, Ogura A.
タイトル Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells.
ジャーナル Biochem Biophys Res Commun
Abstract D1Pas1 is a mouse autosomal DEAD-box RNA helicase expressed predominantly in the testis. To assess its possible function, we generated D1Pas1-deficient mice using embryonic stem cells with a targeted D1Pas1 allele. Deletion of D1Pas1 did not cause noticeable embryonic defects or death, indicating that D1Pas1 is not essential for embryogenesis. Whereas homozygous knockout female mice showed normal reproductive performance, homozygous knockout male mice were completely sterile. The seminiferous epithelium of D1Pas1-deficient males contained no spermatids or spermatozoa because of spermatogenic arrest at the late pachytene stage. Upregulation of retrotransposons such as LINE-1 was not found in D1Pas1-deficient males, unlike males lacking Mvh, another testicular DEAD-box RNA helicase. Meiotic chromosome behavior in developing spermatocytes of D1Pas1-deficient males was indistinguishable from that in wild-type males, at least until synaptonemal complex formation. Thus, mouse D1Pas1 is the first-identified DEAD-box RNA helicase that plays critical roles in the final step of the first meiotic prophase in male germ cells.
巻・号 478(2)
ページ 592-8
公開日 2016-9-16
DOI 10.1016/j.bbrc.2016.07.109
PII S0006-291X(16)31231-1
PMID 27473657
MeSH Animals DEAD-box RNA Helicases / genetics* DEAD-box RNA Helicases / metabolism Female Gene Knockout Techniques Infertility, Male / genetics Infertility, Male / metabolism Male Meiosis* Mice Mice, Inbred C57BL Mice, Knockout Retroelements Spermatocytes / cytology Spermatocytes / metabolism Spermatogenesis*
IF 2.985
引用数 6
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
実験動物マウス RBRC05432