RRC ID 52668
著者 Morisaku T, Yui H.
タイトル Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.
ジャーナル Analyst
Abstract A laser-induced surface deformation (LISD) microscope is developed and applied to measurement of the dynamic relaxation responses of the plasma membrane in a living cell. A laser beam is tightly focused on an optional area of cell surface and the focused light induces microscopic deformation on the surface via radiation pressure. The LISD microscope not only allows non-contact and destruction-free measurement but provides power spectra of the surface responses depending on the frequency of the intensity of the laser beam. An optical system for the LISD is equipped via a microscope, allowing us to measure the relaxation responses in sub-cellular-sized regions of the plasma membrane. In addition, the forced oscillation caused by the radiation pressure for surface deformation extends the upper limit of the frequency range in the obtained power spectra to 106 Hz, which enables us to measure relaxation responses in local regions within the plasma membrane. From differences in power-law exponents at higher frequencies, it is realized that a cancerous cell obeys a weaker single power-law than a normal fibroblast cell. Furthermore, the power spectrum of a keratinocyte cell obeys a power-law with two exponents, indicating that alternative mechanical models to a conventional soft glassy rheology model (where single power-laws explain cells' responses below about 103 Hz) are needed for the understanding over a wider frequency range. The LISD microscope would contribute to investigation of microscopic cell rheology, which is important for clarifying the mechanisms of cell migration and tissue construction.
巻・号 143(10)
ページ 2397-2404
公開日 2018-5-15
DOI 10.1039/c7an01620d
PMID 29700531
MeSH 3T3 Cells Animals Cell Membrane / physiology* Elasticity* HeLa Cells Humans Keratinocytes / physiology Lasers* Mice Pressure Rheology Viscosity*
IF 3.978
引用数 5
リソース情報
ヒト・動物細胞 3T3 Swiss Albino(RCB1642) HeLa(RCB0007)