RRC ID 21566
著者 Watanabe M, Sato M, Kondo K, Narikawa R, Ikeuchi M.
タイトル Phycobilisome model with novel skeleton-like structures in a glaucocystophyte Cyanophora paradoxa.
ジャーナル Biochim Biophys Acta
Abstract Phycobilisome (PBS) is a photosynthetic antenna supercomplex consisting of a central core subcomplex with several peripheral rods radiating from the core. Subunit structure of PBS was studied in a glaucocystophyte Cyanophora paradoxa strain NIES 547. Subunit composition of PBS was identified by N-terminal sequencing and genes for the subunits were determined by homology search of databases. They included rod linker proteins CpcK1 and CpcK2, rod-core linker proteins CpcG1 and CpcG2, and core linker proteins ApcC1 and ApcC2. Subfractionation by native polyacrylamide gel electrophoresis provided evidence for novel subcomplexes (ApcE/CpcK1/CpcG2/ApcA/ApcB/CpcD and ApcE/CpcK2/CpcG1/ApcA/ApcB), which connect rod and core subcomplexes. These skeleton-like structures may serve as a scaffold of the whole PBS assembly. Different roles of ApcC1 and ApcC2 were also suggested. Based on these findings, structural models for PBS were proposed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
巻・号 1817(8)
ページ 1428-35
公開日 2012-8-1
DOI 10.1016/j.bbabio.2011.11.013
PII S0005-2728(11)00285-4
PMID 22172737
MeSH Amino Acid Sequence Centrifugation, Density Gradient Cyanophora / chemistry* Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Phycobilisomes / chemistry* Phylogeny Protein Subunits
IF 3.411
引用数 8
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
藻類 NIES-547