論文 - 詳細
RRC ID | 47431 |
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著者 | Ito H, Sato K, Kondo S, Ueda R, Yamamoto D. |
タイトル | Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila. |
ジャーナル | Curr Biol |
Abstract |
The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robo1) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific Fru(BM) diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C G C C G T G A A in the 5' UTR of robo1 exon1 as the Fru(BM)-responsive element. We find that ∼10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry. |
巻・号 | 26(12) |
ページ | 1532-1542 |
公開日 | 2016-6-20 |
DOI | 10.1016/j.cub.2016.04.067 |
PII | S0960-9822(16)30461-4 |
PMID | 27265393 |
MeSH | Animals Drosophila / growth & development Drosophila / metabolism* Drosophila Proteins / metabolism* Female Male Nerve Tissue Proteins / metabolism* Nervous System / growth & development* Receptors, Immunologic / metabolism* Sex Characteristics* Transcription Factors / metabolism* |
IF | 9.601 |
引用数 | 20 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
ショウジョウバエ |