| RRC ID |
48496
|
| 著者 |
Ueoka N, Kouzuma A, Watanabe K.
|
| タイトル |
Missing Iron-Oxidizing Acidophiles Highly Sensitive to Organic Compounds.
|
| ジャーナル |
Microbes Environ
|
| Abstract |
The genus Acidithiobacillus includes iron-oxidizing lithoautotrophs that thrive in acidic mine environments. Acidithiobacillus ferrooxidans is a representative species and has been extensively studied for its application to the bioleaching of precious metals. In our attempts to cultivate the type strain of A. ferrooxidans (ATCC 23270(T)), repeated transfers to fresh inorganic media resulted in the emergence of cultures with improved growth traits. Strains were isolated from the resultant culture by forming colonies on inorganic silica-gel plates. A representative isolate (strain NU-1) was unable to form colonies on agarose plates and was more sensitive to organics, such as glucose, than the type strain of A. ferrooxidans. Strain NU-1 exhibited superior growth traits in inorganic iron media to those of other iron-oxidizing acidithiobacilli, suggesting its potential for industrial applications. A draft genome of NU-1 uncovered unique features in catabolic enzymes, indicating that this strain is not a mutant of the A. ferrooxidans type strain. Our results indicate that the use of inorganic silica-gel plates facilitates the isolation of as-yet-unexamined iron-oxidizing acidithiobacilli from environmental samples and enrichment cultures.
|
| 巻・号 |
31(3)
|
| ページ |
244-8
|
| 公開日 |
2016-9-29
|
| DOI |
10.1264/jsme2.ME16086
|
| PMID |
27356527
|
| PMC |
PMC5017800
|
| MeSH |
Acidithiobacillus / genetics
Acidithiobacillus / growth & development*
Acidithiobacillus / isolation & purification
Acidithiobacillus / metabolism*
Cluster Analysis
Culture Media / chemistry
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Enzymes / genetics
Genome, Bacterial
Iron / metabolism*
Oxidation-Reduction
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
2.575
|
| 引用数 |
6
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 7812
JCM 18981 |
