RRC ID 15407
著者 Suyari O, Kawai M, Ida H, Yoshida H, Sakaguchi K, Yamaguchi M.
タイトル Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle.
ジャーナル Gene
Abstract In Drosophila, the 255kDa catalytic subunit (dpolεp255) and the 58kDa subunit of DNA polymerase ε (dpolεp58) have been identified. The N-terminus of dpolεp255 carries well-conserved six DNA polymerase subdomains and five 3'→5' exonuclease motifs as observed with Polε in other species. We here examined roles of dpolεp255 during Drosophila development using transgenic fly lines expressing double stranded RNA (dsRNA). Expression of dpolεp255 dsRNA in eye discs induced a small eye phenotype and inhibited DNA synthesis, indicating a role in the G1-S transition and/or S-phase progression of the mitotic cycle. Similarly, expression of dpolεp255 dsRNA in the salivary glands resulted in small size and endoreplication defects, demonstrating a critical role in endocycle progression. In the eye disc, defects induced by knockdown of dpolεp255 were rescued by overexpression of the C-terminal region of dpolεp255, indicating that the function of this non-catalytic domain is conserved between yeast and Drosophila. However, this was not the case for the salivary gland, suggesting that the catalytic N-terminal region is crucial for endoreplication and its defect cannot be complemented by other DNA polymerases. In addition, several genetic interactants with dpolεp255 including genes related to DNA replication such as RFC, DNA primase, DNA polη, Mcm10 and Psf2 and chromatin remodeling such as Iswi were also identified.
巻・号 495(2)
ページ 104-14
公開日 2012-3-10
DOI 10.1016/j.gene.2011.12.056
PII S0378-1119(11)00840-7
PMID 22245183
MeSH Animals Animals, Genetically Modified Catalytic Domain / genetics Cell Cycle / genetics* Cell Cycle Proteins / genetics DNA Polymerase II / genetics* DNA Polymerase II / metabolism* DNA Replication Drosophila / cytology Drosophila / genetics Drosophila / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gene Knockdown Techniques Larva Minichromosome Maintenance Proteins Protein Subunits RNA, Double-Stranded S Phase / genetics Salivary Glands / enzymology
IF 2.984
引用数 10
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ stocks from the Drosophila Genetic Resource Center in Kyoto