RRC ID 36402
著者 Mantoku A, Chatani M, Aono K, Inohaya K, Kudo A.
タイトル Osteoblast and osteoclast behaviors in the turnover of attachment bones during medaka tooth replacement.
ジャーナル Dev Biol
Abstract Tooth replacement in polyphyodont is a well-organized system for maintenance of homeostasis of teeth, containing the dynamic structural change in skeletal tissues such as the attachment bone, which is the supporting element of teeth. Histological analyses have revealed the character of tooth replacement, however, the cellular mechanism of how skeletal tissues are modified during tooth replacement is largely unknown. Here, we showed the important role of osteoblasts for controlling osteoclasts to modify the attachment bone during tooth replacement in medaka pharyngeal teeth, coupled with an osterix-DsRed/TRAP-GFP transgenic line to visualize osteoblasts and osteoclasts. In the turnover of the row of attachment bones, these bones were resorbed at the posterior side where most developed functional teeth were located, and generated at the anterior side where teeth were newly erupted, which caused continuous tooth replacement. In the cellular analysis, osteoclasts and osteoblasts were located at attachment bones separately, since mature osteoclasts were localized at the resorbing side and osteoblasts gathered at the generating side. To demonstrate the role of osteoclasts in tooth replacement, we established medaka made deficient in c-fms-a by TALEN. c-fms-a deficient medaka showed hyperplasia of attachment bones along with reduced bone resorption accompanied by a low number of TRAP-positive osteoclasts, indicating an important role of osteoclasts in the turnover of attachment bones. Furthermore, nitroreductase-mediated osteoblast-specific ablation induced disappearance of osteoclasts, indicating that osteoblasts were essential for maintenance of osteoclasts for the proper turnover. Taken together, our results suggested that the medaka attachment bone provides the model to understand the cellular mechanism for tooth replacement, and that osteoblasts act in the coordination of bone morphology by supporting osteoclasts.
巻・号 409(2)
ページ 370-81
公開日 2016-1-15
DOI 10.1016/j.ydbio.2015.12.002
PII S0012-1606(15)30327-4
PMID 26658319
MeSH Animals Bone Development Bone Remodeling* Bone and Bones / anatomy & histology Bone and Bones / cytology* Larva Mutation / genetics Oryzias Osteoblasts / cytology* Osteoclasts / cytology* Pharynx / anatomy & histology Receptor, Macrophage Colony-Stimulating Factor / metabolism Tooth / anatomy & histology Tooth / physiology* Tooth Germ / cytology
IF 2.896
引用数 9
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
メダカ OK-Cab (MT830) Database osterix-DsRed/TRAP-GFP (TG1359) TRAP-DsRed (TG1362)