RRC ID 48753
著者 Arii Y, Yamaguchi H, Yamasaki M, Fukuoka S.
タイトル Detection of an interaction between prion protein and neuregulin I-β1 by fluorescence resonance energy transfer analysis.
ジャーナル Biosci Biotechnol Biochem
Abstract Cellular prion protein (PrP) copurifies with neuregulin type I-β1 (NRG I-β1), but no interaction has been detected by a general immunoprecipitation study. We speculate that PrP interacts with NRG I-β1. Here, the interaction of PrP with NRG I-β1 was detected by measuring fluorescence resonance energy transfer (FRET) between enhanced blue (EBFP) and enhanced green (EGFP) fluorescent protein-fusion proteins. Full-length PrP interacted with EGFP in addition to NRG I-β1. From this result, we deduced that PrP interacts with EGFP through its unstructured N-terminal domain. We therefore detected FRET between PrP deleting the N-terminal domain and NRG I-β1. In contrast, the C-terminal domain of PrP interacted with NRG I-β1 and the proteins dissociated completely in the presence of sodium chloride. This interaction occurs at the nanomolar level, which is important for the reaction to be functional in organisms. We concluded that PrP interacted with NRG I-β1 through its C-terminal domain.
巻・号 80(4)
ページ 761-8
公開日 2016-1-1
DOI 10.1080/09168451.2015.1116934
PMID 26796243
MeSH Fluorescence Resonance Energy Transfer Neuregulin-1 / metabolism* Prion Proteins / metabolism* Protein Binding
IF 1.516
引用数 0
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY
リソース情報
遺伝子材料 PrP-EBFP (RDB14968) delta N-PrP-EBFB (RDB14969).