RRC ID 30990
著者 Takanashi K, Yokosho K, Saeki K, Sugiyama A, Sato S, Tabata S, Ma JF, Yazaki K.
タイトル LjMATE1: a citrate transporter responsible for iron supply to the nodule infection zone of Lotus japonicus.
ジャーナル Plant Cell Physiol
Abstract Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate.
巻・号 54(4)
ページ 585-94
公開日 2013-4-1
DOI 10.1093/pcp/pct019
PII pct019
PMID 23385147
MeSH Carrier Proteins / genetics Carrier Proteins / metabolism* Iron / metabolism* Lotus / genetics Lotus / metabolism* Lotus / microbiology Molecular Sequence Data Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / genetics Plant Roots / metabolism Plant Roots / microbiology Root Nodules, Plant / genetics Root Nodules, Plant / metabolism* Root Nodules, Plant / microbiology
IF 4.062
引用数 42
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
ミヤコグサ・ダイズ Miyakojima MG-20 pUB-GWS-GFP/Hyg