RRC ID 48442
著者 Ng LM, Sudesh K.
タイトル Identification of a new polyhydroxyalkanoate (PHA) producer Aquitalea sp. USM4 (JCM 19919) and characterization of its PHA synthase.
ジャーナル J Biosci Bioeng
Abstract Aquitalea sp. USM4 (JCM 19919) was isolated from a freshwater sample at Lata Iskandar Waterfall in Perak, Malaysia. It is a rod-shaped, gram-negative bacterium with high sequence identity (99%) to Aquitalea magnusonii based on 16S rRNA gene analysis. Aquitalea sp. USM4 also possessed a PHA synthase gene (phaC), which had amino acid sequence identity of 77-78% to the PHA synthase of Chromobacterium violaceum ATCC12472 and Pseudogulbenkiania sp. NH8B. PHA biosynthesis results showed that wild-type Aquitalea sp. USM4 was able to accumulate up to 1.5 g/L of poly(3-hydroxybutyrate), [P(3HB)]. The heterologous expression of the PHA synthase gene of Aquitalea sp. USM4 (phaCAq) in Cupriavidus necator PHB-4 had resulted in PHA accumulation up to 3.2 g/L of P(3HB). It was further confirmed by 1H nuclear magnetic resonance (NMR) analysis that Aquitalea sp. USM4 and C. necator PHB-4 transformant were able to produce PHA containing 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB) and 3-hydroxy-4-methylvalerate (3H4MV) monomers from suitable precursor substrates. Interestingly, relatively high PHA synthase activity of 863 U/g and 1402 U/g were determined in wild-type Aquitalea sp. USM4 and C. necator PHB-4 transformant respectively. This is the first report on the member of genus Aquitalea as a new PHA producer as well as in vitro and in vivo characterization of a novel PHA synthase from Aquitalea sp. USM4.
巻・号 122(5)
ページ 550-557
公開日 2016-11-1
DOI 10.1016/j.jbiosc.2016.03.024
PII S1389-1723(16)30046-9
PMID 27132174
MeSH Acyltransferases / genetics* Acyltransferases / isolation & purification Acyltransferases / metabolism* Amino Acid Sequence Chromobacterium / enzymology* Chromobacterium / genetics Chromobacterium / isolation & purification* Chromobacterium / metabolism Cloning, Molecular Cupriavidus necator / enzymology Cupriavidus necator / genetics Cupriavidus necator / metabolism Fresh Water / microbiology Pentanoic Acids / metabolism Phylogeny Polyesters / metabolism Polyhydroxyalkanoates / biosynthesis* RNA, Ribosomal, 16S / metabolism Sequence Analysis, DNA
IF 2.366
引用数 9
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
一般微生物 JCM 19919