| RRC ID |
33626
|
| 著者 |
Wai HA, Kawakami K, Wada H, Müller F, Vernallis AB, Brown G, Johnson WE.
|
| タイトル |
The development and growth of tissues derived from cranial neural crest and primitive mesoderm is dependent on the ligation status of retinoic acid receptor γ: evidence that retinoic acid receptor γ functions to maintain stem/progenitor cells in the absence of retinoic acid.
|
| ジャーナル |
Stem Cells Dev
|
| Abstract |
Retinoic acid (RA) signaling is important to normal development. However, the function of the different RA receptors (RARs)--RARα, RARβ, and RARγ--is as yet unclear. We have used wild-type and transgenic zebrafish to examine the role of RARγ. Treatment of zebrafish embryos with an RARγ-specific agonist reduced somite formation and axial length, which was associated with a loss of hoxb13a expression and less-clear alterations in hoxc11a or myoD expression. Treatment with the RARγ agonist also disrupted formation of tissues arising from cranial neural crest, including cranial bones and anterior neural ganglia. There was a loss of Sox 9-immunopositive neural crest stem/progenitor cells in the same anterior regions. Pectoral fin outgrowth was blocked by RARγ agonist treatment. However, there was no loss of Tbx-5-immunopositive lateral plate mesodermal stem/progenitor cells and the block was reversed by agonist washout or by cotreatment with an RARγ antagonist. Regeneration of the caudal fin was also blocked by RARγ agonist treatment, which was associated with a loss of canonical Wnt signaling. This regenerative response was restored by agonist washout or cotreatment with the RARγ antagonist. These findings suggest that RARγ plays an essential role in maintaining stem/progenitor cells during embryonic development and tissue regeneration when the receptor is in its nonligated state.
|
| 巻・号 |
24(4)
|
| ページ |
507-19
|
| 公開日 |
2015-2-15
|
| DOI |
10.1089/scd.2014.0235
|
| PMID |
25233141
|
| PMC |
PMC4313414
|
| MeSH |
Animals
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / metabolism
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism
Neural Crest / cytology
Neural Crest / embryology
Neural Crest / metabolism*
Neurogenesis
Osteogenesis
Receptors, Retinoic Acid / agonists
Receptors, Retinoic Acid / antagonists & inhibitors
Receptors, Retinoic Acid / metabolism*
SOX9 Transcription Factor / metabolism
Somites / cytology
Somites / embryology
Somites / metabolism*
T-Box Domain Proteins / metabolism
Tretinoin / metabolism
Wnt Signaling Pathway
Zebrafish
|
| IF |
3.153
|
| 引用数 |
6
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
TRANSPLANTATION
HEMATOLOGY
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| ゼブラフィッシュ |
HGn39D
hspGFFDMC28B
hspGFF55B
SAGFF(LF)155A? |
