RRC ID 3825
著者 Nakamura S, Aoki Y, Saito D, Kuroki Y, Fujiyama A, Naruse K, Tanaka M.
タイトル Sox9b/sox9a2-EGFP transgenic medaka reveals the morphological reorganization of the gonads and a common precursor of both the female and male supporting cells.
ジャーナル Mol Reprod Dev
Abstract We have established an enhanced green fluorescent protein (EGFP) transgenic medaka line that mimics the expression of sox9b/sox9a2 to analyze the morphological reorganization of the gonads and characterize the sox9b-expressing cells during gonadal formation in this fish. After the germ cells have migrated into the gonadal areas, a cluster of EGFP-expressing cells in the single gonadal primordium was found to be separated by the somatic cells along the rostrocaudal axis and form the bilateral lobes. We observed in these transgenic fish that EGFP expression persists only in the somatic cells directly surrounding the germ cells. As sex differentiation proceeds, dmrt1 and foxl2 begin to be expressed in the EGFP-expressing cells in the XY and the XX gonads, respectively. This indicates that the sox9b-expressing cells reorganize into two lobes of the gonad and then differentiate into Sertoli or granulosa cells, as common precursors of the supporting cells. Hence, our sox9b-EGFP medaka system will be useful in future studies of gonadal development.
巻・号 75(3)
ページ 472-6
公開日 2008-3-1
DOI 10.1002/mrd.20764
PMID 17474097
MeSH Animals Animals, Genetically Modified Embryo, Nonmammalian Female Gene Expression Regulation, Developmental Gonads / embryology* Gonads / metabolism Granulosa Cells / cytology* Granulosa Cells / metabolism Granulosa Cells / physiology Green Fluorescent Proteins / genetics* Green Fluorescent Proteins / metabolism High Mobility Group Proteins / genetics* High Mobility Group Proteins / metabolism Male Oryzias / embryology Oryzias / genetics* Protein Isoforms / genetics Protein Isoforms / metabolism SOX9 Transcription Factor Sertoli Cells / cytology* Sertoli Cells / metabolism Sertoli Cells / physiology Transcription Factors / genetics* Transcription Factors / metabolism
IF 2.823
引用数 55
WOS 分野 REPRODUCTIVE BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
メダカ BAC (ola1-014G01) inbred cab strain