RRC ID 36219
著者 Zabrady K, Adamus M, Vondrova L, Liao C, Skoupilova H, Novakova M, Jurcisinova L, Alt A, Oliver AW, Lehmann AR, Palecek JJ.
タイトル Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA.
ジャーナル Nucleic Acids Res
Abstract SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and processing. However, the precise mechanism of action of SMC5/6 is not known. Here we present evidence that the NSE1/NSE3/NSE4 sub-complex of SMC5/6 binds to double-stranded DNA without any preference for DNA-replication/recombination intermediates. Mutations of key basic residues within the NSE1/NSE3/NSE4 DNA-binding surface reduce binding to DNA in vitro. Their introduction into the Schizosaccharomyces pombe genome results in cell death or hypersensitivity to DNA damaging agents. Chromatin immunoprecipitation analysis of the hypomorphic nse3 DNA-binding mutant shows a reduced association of fission yeast SMC5/6 with chromatin. Based on our results, we propose a model for loading of the SMC5/6 complex onto the chromatin.
巻・号 44(3)
ページ 1064-79
公開日 2016-2-18
DOI 10.1093/nar/gkv1021
PII gkv1021
PMID 26446992
PMC PMC4756808
MeSH Amino Acid Sequence Cell Cycle Proteins / chemistry Cell Cycle Proteins / metabolism* Chromatin / metabolism* DNA / metabolism* DNA Replication Humans Molecular Sequence Data Nuclear Proteins / metabolism* Protein Binding Recombination, Genetic Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / metabolism* Sequence Homology, Amino Acid
IF 11.502
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY18537