RRC ID 45563
著者 Gerhold AR, Ryan J, Vallée-Trudeau JN, Dorn JF, Labbé JC, Maddox PS.
タイトル Investigating the regulation of stem and progenitor cell mitotic progression by in situ imaging.
ジャーナル Curr Biol
Abstract Genome stability relies upon efficacious chromosome congression and regulation by the spindle assembly checkpoint (SAC). The study of these fundamental mitotic processes in adult stem and progenitor cells has been limited by the technical challenge of imaging mitosis in these cells in situ. Notably, how broader physiological changes, such as dietary intake or age, affect mitotic progression in stem and/or progenitor cells is largely unknown. Using in situ imaging of C. elegans adult germlines, we describe the mitotic parameters of an adult stem and progenitor cell population in an intact animal. We find that SAC regulation in germline stem and progenitor cells is distinct from that found in early embryonic divisions and is more similar to that of classical tissue culture models. We further show that changes in organismal physiology affect mitotic progression in germline stem and progenitor cells. Reducing dietary intake produces a checkpoint-dependent delay in anaphase onset, and inducing dietary restriction when the checkpoint is impaired increases the incidence of segregation errors in mitotic and meiotic cells. Similarly, developmental aging of the germline stem and progenitor cell population correlates with a decline in the rate of several mitotic processes. These results provide the first in vivo validation of models for SAC regulation developed in tissue culture systems and demonstrate that several fundamental features of mitotic progression in adult stem and progenitor cells are highly sensitive to organismal physiological changes.
巻・号 25(9)
ページ 1123-34
公開日 2015-5-4
DOI 10.1016/j.cub.2015.02.054
PII S0960-9822(15)00223-7
PMID 25819563
MeSH Adult Stem Cells / physiology* Animals Caenorhabditis elegans Food Deprivation Green Fluorescent Proteins Homeostasis M Phase Cell Cycle Checkpoints Mitosis*
IF 9.601
引用数 16
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm2190