RRC ID 51396
著者 Spracklin G, Fields B, Wan G, Becker D, Wallig A, Shukla A, Kennedy S.
タイトル The RNAi Inheritance Machinery of Caenorhabditis elegans.
ジャーナル Genetics
Abstract Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel nematode-specific factors that we term here (heritable RNAi defective) HRDE-2 and HRDE-4 The new RNAi inheritance factors exhibit mortal germline (Mrt) phenotypes, which we show is likely caused by epigenetic deregulation in germ cells. We also show that HRDE-2 contributes to RNAi inheritance by facilitating the binding of small RNAs to the inheritance Argonaute (Ago) HRDE-1 Together, our results identify additional components of the RNAi inheritance machinery whose conservation provides insights into the molecular mechanism of RNAi inheritance, further our understanding of how the RNAi inheritance machinery promotes germline immortality, and show that HRDE-2 couples the inheritance Ago HRDE-1 with the small RNAs it needs to direct RNAi inheritance and germline immortality.
巻・号 206(3)
ページ 1403-1416
公開日 2017-7-1
DOI 10.1534/genetics.116.198812
PII genetics.116.198812
PMID 28533440
PMC PMC5500139
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Epigenesis, Genetic Gene Silencing* Phenotype RNA, Small Interfering / genetics*
IF 4.015
引用数 35
リソース情報
線虫 tm1200 tm1358 tm6048