RRC ID 30084
著者 Young KK, Edlin G.
タイトル Physical and genetical analysis of bacteriophage T4 generalized transduction.
ジャーナル Mol Gen Genet
Abstract This report describes a comparison of the efficiency of transduction of genes in E. coli by the generalized transducing bacteriophages T4GT7 and P1CM. Both phages are capable of transducing many genetic markers in E. coli although the frequency of transduction for particular genes varies over a wide range. The frequency of transduction for most genes depends on which transducing phage is used as well as on the donor and recipient bacterial strains. Analysis of T4GT7 phage lysates by cesium chloride density gradient centrifugation shows that transducing phage particles contain primarily bacterial DNA and carry little, if any, phage DNA. In this regard transducing phages P1CM and T4GT7 are similar; both phages package either bacterial or phage DNA but not both DNAs into the same particle.
巻・号 192(1-2)
ページ 241-6
公開日 1983-1-1
DOI 10.1007/BF00327673
PMID 6358795
MeSH Coliphages / genetics DNA, Bacterial / genetics Escherichia coli / genetics* Genes, Bacterial T-Phages / genetics* Transduction, Genetic*
引用数 30
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME6040