RRC ID 50307
著者 Myers TR, Amendola PG, Lussi YC, Salcini AE.
タイトル JMJD-1.2 controls multiple histone post-translational modifications in germ cells and protects the genome from replication stress.
ジャーナル Sci Rep
Abstract Post-translational modifications of histones, constitutive components of chromatin, regulate chromatin compaction and control all DNA-based cellular processes. C. elegans JMJD-1.2, a member of the KDM7 family, is a demethylase active towards several lysine residues on Histone 3 (H3), but its contribution in regulating histone methylation in germ cells has not been fully investigated. Here, we show that jmjd-1.2 is expressed abundantly in the germline where it controls the level of histone 3 lysine 9, lysine 23 and lysine 27 di-methylation (H3K9/K23/K27me2) both in mitotic and meiotic cells. Loss of jmjd-1.2 is not associated with major defects in the germ cells in animals grown under normal conditions or after DNA damage induced by UV or ionizing irradiation. However, jmjd-1.2 mutants are more sensitive to replication stress and the progeny of mutant animals exposed to hydroxyurea show increased embryonic lethality and mutational rate, compared to wild-type. Thus, our results suggest a role for jmjd-1.2 in the maintenance of genome integrity after replication stress and emphasize the relevance of the regulation of histone methylation in genomic stability.
巻・号 8(1)
ページ 3765
公開日 2018-2-28
DOI 10.1038/s41598-018-21914-9
PII 10.1038/s41598-018-21914-9
PMID 29491442
PMC PMC5830613
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA Damage / drug effects* DNA Replication / genetics* Genomic Instability Germ Cells / metabolism* Histones / metabolism* Jumonji Domain-Containing Histone Demethylases / genetics Jumonji Domain-Containing Histone Demethylases / metabolism* Mutation Protein Processing, Post-Translational* Protein Transport
IF 3.998
引用数 3
リソース情報
線虫 jmjd-1.2(tm3713)