RRC ID 45651
著者 Vandamme J, Sidoli S, Mariani L, Friis C, Christensen J, Helin K, Jensen ON, Salcini AE.
タイトル H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans.
ジャーナル Nucleic Acids Res
Abstract Genome-wide analyses in Caenorhabditis elegans show that post-translational modifications (PTMs) of histones are evolutionary conserved and distributed along functionally distinct genomic domains. However, a global profile of PTMs and their co-occurrence on the same histone tail has not been described in this organism. We used mass spectrometry based middle-down proteomics to analyze histone H3 N-terminal tails from C. elegans embryos for the presence, the relative abundance and the potential cross-talk of co-existing PTMs. This analysis highlighted that the lysine 23 of histone H3 (H3K23) is extensively modified by methylation and that tri-methylated H3K9 (H3K9me3) is exclusively detected on histone tails with di-methylated H3K23 (H3K23me2). Chromatin immunoprecipitation approaches revealed a positive correlation between H3K23me2 and repressive marks. By immunofluorescence analyses, H3K23me2 appears differentially regulated in germ and somatic cells, in part by the action of the histone demethylase JMJD-1.2. H3K23me2 is enriched in heterochromatic regions, localizing in H3K9me3 and heterochromatin protein like-1 (HPL-1)-positive foci. Biochemical analyses indicated that HPL-1 binds to H3K23me2 and interacts with a conserved CoREST repressive complex. Thus, our study suggests that H3K23me2 defines repressive domains and contributes to organizing the genome in distinct heterochromatic regions during embryogenesis.
巻・号 43(20)
ページ 9694-710
公開日 2015-11-16
DOI 10.1093/nar/gkv1063
PII gkv1063
PMID 26476455
PMC PMC4787770
MeSH Acetylation Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Chromosomal Proteins, Non-Histone / analysis Co-Repressor Proteins / metabolism Embryo, Nonmammalian / metabolism Embryonic Development Germ Cells / metabolism Heterochromatin / metabolism* Histone Code* Histone Demethylases / metabolism Histones / analysis Histones / chemistry Histones / metabolism* Lysine / metabolism Methylation Protein Processing, Post-Translational
IF 11.502
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm3713 tm1489 tm4586