RRC ID 49304
著者 Luo L, Siah CK, Cai Y.
タイトル Engrailed acts with Nejire to control decapentaplegic expression in the Drosophila ovarian stem cell niche.
ジャーナル Development
Abstract Homeostasis of adult tissues is maintained by a small number of stem cells, which are sustained by their niches. In the Drosophila female germline stem cell (GSC) niche, Decapentaplegic (Dpp) is the primary factor that promotes GSC self-renewal. However, the mechanism regulating dpp expression in the niche is largely unknown. Here, we identify a 2.0 kb fragment located in a 5' cis-regulatory region of the dpp locus containing enhancer activity that drives its expression in the niche. This region is distinct from a previously characterized 3' cis-regulatory enhancer responsible for dpp expression in imaginal discs. Our data demonstrate that Engrailed, a homeodomain-containing transcription factor that serves as a cap cell marker, binds to this region and regulates dpp expression in cap cells. Further data suggest that En forms a complex with Nejire (Nej), the Drosophila ortholog of histone acetyltransferase CBP/p300, and directs Nej to this cis-regulatory region where Nej functions as the co-activator for dpp expression. Therefore, our study defines the molecular pathway controlling dpp expression in the Drosophila ovarian stem cell niche.
巻・号 144(18)
ページ 3224-3231
公開日 2017-9-15
DOI 10.1242/dev.145474
PII 144/18/3224
PMID 28928281
MeSH Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Female Gene Expression Regulation Genes, Reporter Homeodomain Proteins / metabolism* Ovary / cytology* Ovary / metabolism* Protein Binding Regulatory Sequences, Nucleic Acid / genetics Stem Cell Niche* / genetics Stem Cells / cytology Stem Cells / metabolism Transcription Factors / metabolism* p300-CBP Transcription Factors / metabolism*
IF 5.611
引用数 6
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