RRC ID 47914
著者 Kenmoku H, Hori A, Kuraishi T, Kurata S.
タイトル A novel mode of induction of the humoral innate immune response in Drosophila larvae.
ジャーナル Dis Model Mech
Abstract Drosophila adults have been utilized as a genetically tractable model organism to decipher the molecular mechanisms of humoral innate immune responses. In an effort to promote the utility of Drosophila larvae as an additional model system, in this study, we describe a novel aspect of an induction mechanism for innate immunity in these larvae. By using a fine tungsten needle created for manipulating semi-conductor devices, larvae were subjected to septic injury. However, although Toll pathway mutants were susceptible to infection with Gram-positive bacteria as had been shown for Drosophila adults, microbe clearance was not affected in the mutants. In addition, Drosophila larvae were found to be sensitive to mechanical stimuli with respect to the activation of a sterile humoral response. In particular, pinching with forceps to a degree that might cause minor damage to larval tissues could induce the expression of the antifungal peptide gene Drosomycin; notably, this induction was partially independent of the Toll and immune deficiency pathways. We therefore propose that Drosophila larvae might serve as a useful model to analyze the infectious and non-infectious inflammation that underlies various inflammatory diseases such as ischemia, atherosclerosis and cancer.
巻・号 10(3)
ページ 271-281
公開日 2017-3-1
DOI 10.1242/dmm.027102
PII 10/3/271
PMID 28250052
PMC PMC5374318
MeSH Animals Antimicrobial Cationic Peptides / metabolism Down-Regulation / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / immunology* Drosophila melanogaster / microbiology Gram-Positive Bacterial Infections / immunology Gram-Positive Bacterial Infections / microbiology Gram-Positive Bacterial Infections / pathology Hemocytes / metabolism Immunity, Humoral* Immunity, Innate* Larva / immunology Larva / microbiology Mutation / genetics Sensory Receptor Cells / metabolism Signal Transduction / genetics Up-Regulation / genetics
IF 4.651
引用数 17
WOS 分野 PATHOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ CAS-0001