論文 - 詳細
| RRC ID | 253 |
|---|---|
| 著者 | Scholz S, Domaschke H, Kanamori A, Ostermann K, Rödel G, Gutzeit HO. |
| タイトル | Germ cell-less expression in medaka (Oryzias latipes). |
| ジャーナル | Mol Reprod Dev |
| Abstract |
The gene germ cell-less (gcl) plays an important role in the early differentiation of germ cells in Drosophila. We isolated the gcl homolog of the model teleost medaka (Oryzias latipes) using degenerated primers and an ovary cDNA bank. The predicted amino acid sequence of medaka gcl showed 92, 68 and 31% overall identity to mouse, human and Drosophila gcl respectively. RT-PCR revealed stronger expression in the ovary and weaker expression in testis, brain, heart, liver and muscle tissue. Expression in early embryos indicates the presence of maternal mRNA. By in situ hybridisation (ISH), gcl could not be detected in embryos. In contrast to vasa, ISH revealed expression of gcl in the ovary but not in the testis. Mol. Reprod. Dev. 67: 15-18, 2004. |
| 巻・号 | 67(1) |
| ページ | 15-8 |
| 公開日 | 2004-1-1 |
| DOI | 10.1002/mrd.20012 |
| PMID | 14648871 |
| MeSH | Amino Acid Sequence Animals Cell Differentiation / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism* Female Germ Cells / cytology Germ Cells / physiology* Humans In Situ Hybridization Intercellular Signaling Peptides and Proteins Male Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism* Oryzias / anatomy & histology Oryzias / embryology Oryzias / physiology* Ovary / cytology Ovary / metabolism Sequence Alignment Sequence Homology, Amino Acid Testis / cytology Testis / metabolism Tissue Distribution |
| IF | 2.823 |
| 引用数 | 6 |
| WOS 分野 | REPRODUCTIVE BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| メダカ | d-rR/NAGOYA (MT847) |