RRC ID 28112
著者 Bjerkan KN, Jung-Roméo S, Jürgens G, Genschik P, Grini PE.
タイトル Arabidopsis WD repeat domain55 Interacts with DNA damaged binding protein1 and is required for apical patterning in the embryo.
ジャーナル Plant Cell
Abstract CUL4-RING ubiquitin E3 ligases (CRL4s) were recently shown to exert their specificity through the binding of various substrate receptors, which bind the CUL4 interactor DNA damaged binding protein1 (DDB1) through a WDxR motif. In a segregation-based mutagenesis screen, we identified a WDxR motif-containing protein (WDR55) required for male and female gametogenesis and seed development. We demonstrate that WDR55 physically interacts with Arabidopsis thaliana DDB1A in planta, suggesting that WDR55 may be a novel substrate recruiter of CRL4 complexes. Examination of mutants revealed a failure in the fusion of the polar cells in embryo sac development, in addition to embryo and endosperm developmental arrest at various stages ranging from the zygote stage to the globular stage. wdr55-2 embryos suggest a defect in the transition to bilateral symmetry in the apical embryo domain, further supported by aberrant apical embryo localization of DORNROESCHEN, a direct target of the auxin response factor protein monopteros. Moreover, the auxin response pattern, as determined using the synthetic auxin-responsive reporter ProDR5:green fluorescent protein, was shifted in the basal embryo and suspensor but does not support a strong direct link to auxin response. Interestingly, the observed embryo and endosperm phenotype is reminiscent of CUL4 or DDB1A/B loss of function and thus may support a regulatory role of a putative CRL4(WDR55) E3 ligase complex.
巻・号 24(3)
ページ 1013-33
公開日 2012-3-1
DOI 10.1105/tpc.111.089425
PII tpc.111.089425
PMID 22447688
PMC PMC3336142
MeSH Amino Acid Sequence Arabidopsis / embryology Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cloning, Molecular Cullin Proteins / metabolism Endosperm / embryology Endosperm / genetics Gametogenesis, Plant* Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genetic Complementation Test Molecular Sequence Data Mutation Seeds / embryology* Seeds / genetics
IF 9.618
引用数 22
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda20340