RRC ID 41207
著者 Zhao J, Guo L, Sun P, Han C, Bai L, Liu C, Li Y, Xiang W, Wang X.
タイトル Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov., two novel actinomycetes isolated from muddy soil and emended description of the genus Actinomadura.
ジャーナル Antonie Van Leeuwenhoek
Abstract Two novel actinomycetes, designated strains NEAU-Jh1-3(T) and NEAU-Jh2-5(T), were isolated from muddy soil collected from a riverbank in Jiaohe, Jilin Province, north China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates exhibited 99.9 % 16S rRNA gene sequence similarity with each other and that they are closely related to Actinomadura viridis IFO 15238(T) (99.6, 99.6 %) and Actinomadura vinacea IFO 14688(T) (99.3, 99.3 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two cultures clustered together and formed a cluster with A. viridis IFO 15238(T), A. vinacea IFO 14688(T) and Actinomadura rugatobispora IFO 14382(T). However, the DNA-DNA hybridization value between strains NEAU-Jh1-3(T) and NEAU-Jh2-5(T) was 63.6 %, and the values between the two strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the two strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-Jh1-3(T) and NEAU-Jh2-5(T) represent two novel species of the genus Actinomadura, for which the names Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov. are proposed. The type strains are NEAU-Jh1-3(T) (=CGMCC 4.7197(T) = JCM 30341(T)) and NEAU-Jh2-5(T) (=CGMCC 4.7211(T) = JCM 30342(T)), respectively.
巻・号 108(6)
ページ 1331-1339
公開日 2015-12-1
DOI 10.1007/s10482-015-0585-1
PII 10.1007/s10482-015-0585-1
PMID 26373415
MeSH Actinobacteria / classification* Actinobacteria / genetics Actinobacteria / isolation & purification* Actinobacteria / physiology Bacterial Typing Techniques China Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Microscopy, Electron, Scanning Molecular Sequence Data Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology*
IF 2.674
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30341 JCM 30342