RRC ID 48158
著者 Guo X, Zhou S, Wang YW, Wang HM, Kong DL, Zhu J, Dong WW, He MX, Zhao BQ, Hu GQ, Ruan ZY.
タイトル Paenibacillus salinicaeni sp. nov., isolated from saline silt sample.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel facultatively anaerobic bacterium, designated strain LAM0A28(T), was isolated from a saline silt sample collected from the Chinese Sea of Death located in Suining city, Sichuan province, China. Cells of strain LAM0A28(T) were observed to be Gram-stain positive, motile, endospore-forming and straight-rod shaped. Strain LAM0A28(T) was found to be able to grow at 15-45 °C (optimum: 30-35 °C), pH 5.0-10.0 (optimum: 7.5) and 0-5 % NaCl (w/v) (optimum: 0.5 %). The 16S rRNA gene sequence similarity analysis showed that strain LAM0A28(T) is closely related to Paenibacillus jilunlii DSM 23019(T) (97.5 %) and Paenibacillus graminis DSM 15220(T) (97.2 %). The DNA-DNA hybridization values between the isolate and P. jilunlii DSM 23019(T), P. graminis DSM 15220(T) were 30.2 ± 1.6 % and 44.7 ± 2.1 %, respectively. The DNA G+C content was found to be 51.2 mol% as determined by the T m method. The major cellular fatty acids were identified as anteiso-C15:0, C16:0, iso-C16:0 and C14:0. The major isoprenoid quinone was identified as MK-7. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids and six unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM0A28(T) is concluded to represent a novel species within the genus Paenibacillus, for which the name Paenibacillus salinicaeni sp. nov. is proposed. The type strain is LAM0A28(T) (=ACCC 00741(T) = JCM 30850(T)).
巻・号 109(5)
ページ 721-8
公開日 2016-5-1
DOI 10.1007/s10482-016-0674-9
PII 10.1007/s10482-016-0674-9
PMID 26979511
MeSH Anaerobiosis Bacterial Typing Techniques China DNA, Bacterial / genetics DNA, Ribosomal / genetics Paenibacillus / classification* Paenibacillus / genetics Paenibacillus / isolation & purification* Paenibacillus / physiology Phylogeny Salinity Seawater / microbiology* Spores, Bacterial / cytology
IF 2.674
引用数 6
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30850