RRC ID |
30245
|
著者 |
Aihara Y, Yasuoka A, Yoshida Y, Ohmoto M, Shimizu-Ibuka A, Misaka T, Furutani-Seiki M, Matsumoto I, Abe K.
|
タイトル |
Transgenic labeling of taste receptor cells in model fish under the control of the 5'-upstream region of medaka phospholipase C-beta 2 gene.
|
ジャーナル |
Gene Expr Patterns
|
Abstract |
Vertebrate taste receptor cells express signaling molecules such as taste receptors and effectors to convert taste stimuli to inner cellular signals. Phospholipase C-beta2 (PLC-beta2) is an effector enzyme that is necessary to transduce taste signals in the mouse. It was shown that a subset of the plc-beta2 expressing cells also express taste receptor molecules, T1Rs or T2Rs, in mammals and fish. To label plc-beta2 expressing cells in the model fish species, we constructed a transgene by linking the 5'-upstream region of the medaka plc-beta2 gene to a green fluorescent protein (GFP) gene. The resulting transgenic medaka exhibited GFP signals in taste buds of the lips and the pharyngeal region. Detailed observation revealed that the GFP signals were in a subpopulation of taste bud cells, and co-localized with the transcript of endogenous plc-beta2 gene. Zebrafish introduced with the same transgene showed GFP signals in a subpopulation of taste bud cells of the lips and the pharyngeal region as in the case of medaka. This is the first report of successful labeling of taste receptor cells in two model fish species under the control of the plc-beta2 promoter. This promoter will be a useful genetic tool to study the vertebrate taste system in general.
|
巻・号 |
7(1-2)
|
ページ |
149-57
|
公開日 |
2007-1-1
|
DOI |
10.1016/j.modgep.2006.06.004
|
PII |
S1567-133X(06)00112-8
|
PMID |
16920036
|
MeSH |
Animals
Animals, Genetically Modified
Base Sequence
DNA Primers / genetics
Green Fluorescent Proteins / genetics
In Situ Hybridization
Isoenzymes / genetics*
Models, Genetic
Oryzias / genetics*
Oryzias / metabolism
Phospholipase C beta
Promoter Regions, Genetic
Recombinant Proteins / genetics
Taste Buds / cytology
Taste Buds / metabolism*
Type C Phospholipases / genetics*
Zebrafish / genetics
Zebrafish / metabolism
|
IF |
0.897
|
引用数 |
13
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
ゼブラフィッシュ |
RIKEN WT |
メダカ |
cab
Cab-Tg(mfplcb2-GFP) (TG852) |