RRC ID 27313
著者 Yamashita A, Takayama T, Iwata R, Yamamoto M.
タイトル A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts.
ジャーナル Nucleic Acids Res
Abstract A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces nuclear exosome-mediated elimination. During meiosis, Mmi1 is sequestered by a chromosome-associated dot structure, Mei2 dot, allowing meiosis-specific transcripts to be stably expressed. Red1, a zinc-finger protein, is also known to participate in the Mmi1/DSR elimination system, although its molecular function has remained elusive. To uncover the detailed molecular mechanisms underlying the Mmi1/DSR elimination system, we sought to identify factors that interact genetically with Mmi1. Here, we show that one of the identified factors, Iss10, is involved in the Mmi1/DSR system by regulating the interaction between Mmi1 and Red1. In cells lacking Iss10, association of Red1 with Mmi1 is severely impaired, and target transcripts of Mmi1 are ectopically expressed in the mitotic cycle. During meiosis, Iss10 is downregulated, resulting in dissociation of Red1 from Mmi1 and subsequent suppression of Mmi1 activity.
巻・号 41(21)
ページ 9680-7
公開日 2013-11-1
DOI 10.1093/nar/gkt763
PII gkt763
PMID 23980030
PMC PMC3834831
MeSH Cell Cycle Proteins / analysis Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Gene Expression Regulation, Fungal* Meiosis / genetics* RNA, Messenger / metabolism* Schizosaccharomyces / chemistry Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / analysis Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* mRNA Cleavage and Polyadenylation Factors / analysis mRNA Cleavage and Polyadenylation Factors / metabolism*
IF 11.502
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 FY13690(JV393) FY24020(JY450) FY13307(JZ462) FY13309(JZ464) FY7716(JZ670)