RRC ID |
50002
|
著者 |
Hirano N, Marukawa Y, Abe J, Hashiba S, Ichikawa M, Tanabe Y, Ito M, Nishii I, Tsuchikane Y, Sekimoto H.
|
タイトル |
A Receptor-Like Kinase, Related to Cell Wall Sensor of Higher Plants, is Required for Sexual Reproduction in the Unicellular Charophycean Alga, Closterium peracerosum-strigosum-littorale Complex.
|
ジャーナル |
Plant Cell Physiol
|
Abstract |
Here, we cloned the CpRLK1 gene, which encodes a receptor-like protein kinase expressed during sexual reproduction, from the heterothallic Closterium peracerosum-strigosum-littorale complex, one of the closest unicellular alga to land plants. Mating-type plus (mt(+)) cells with knockdown of CpRLK1 showed reduced competence for sexual reproduction and formed an abnormally enlarged conjugation papilla after pairing with mt(-) cells. The knockdown cells were unable to release a naked gamete, which is indispensable for zygote formation. We suggest that the CpRLK1 protein is an ancient cell wall sensor that now functions to regulate osmotic pressure in the cell to allow proper gamete release.
|
巻・号 |
56(7)
|
ページ |
1456-62
|
公開日 |
2015-7-1
|
DOI |
10.1093/pcp/pcv065
|
PII |
pcv065
|
PMID |
25941232
|
MeSH |
Algal Proteins / classification
Algal Proteins / genetics*
Algal Proteins / metabolism
Amino Acid Sequence
Cell Wall / genetics
Cell Wall / metabolism
Cloning, Molecular
Closterium / genetics*
Closterium / metabolism
Closterium / physiology
DNA, Complementary / chemistry
DNA, Complementary / genetics
Gene Knockdown Techniques
Immunoblotting
Microscopy, Confocal
Molecular Sequence Data
Osmotic Pressure / physiology
Phylogeny
Plants / genetics
Plants / metabolism
Protein Kinases / classification
Protein Kinases / genetics*
Protein Kinases / metabolism
Reproduction / genetics
Reproduction / physiology
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Time-Lapse Imaging / methods
|
IF |
4.062
|
引用数 |
10
|
リソース情報 |
藻類 |
NIES-67
NIES-68 |