RRC ID 35152
著者 Otani T, Oshima K, Kimpara A, Takeda M, Abdu U, Hayashi S.
タイトル A transport and retention mechanism for the sustained distal localization of Spn-F-IKKε during Drosophila bristle elongation.
ジャーナル Development
Abstract Stable localization of the signaling complex is essential for the robust morphogenesis of polarized cells. Cell elongation involves molecular signaling centers that coordinately regulate intracellular transport and cytoskeletal structures. In Drosophila bristle elongation, the protein kinase IKKε is activated at the distal tip of the growing bristle and regulates the shuttling movement of recycling endosomes and cytoskeletal organization. However, how the distal tip localization of IKKε is established and maintained during bristle elongation is unknown. Here, we demonstrate that IKKε distal tip localization is regulated by Spindle-F (Spn-F), which is stably retained at the distal tip and functions as an adaptor linking IKKε to cytoplasmic dynein. We found that Javelin-like (Jvl) is a key regulator of Spn-F retention. In jvl mutant bristles, IKKε and Spn-F initially localize to the distal tip but fail to be retained there. In S2 cells, particles that stain positively for Jvl or Spn-F move in a microtubule-dependent manner, whereas Jvl and Spn-F double-positive particles are immobile, indicating that Jvl and Spn-F are transported separately and, upon forming a complex, immobilize each other. These results suggest that polarized transport and selective retention regulate the distal tip localization of the Spn-F-IKKε complex during bristle cell elongation.
巻・号 142(13)
ページ 2338-51
公開日 2015-7-1
DOI 10.1242/dev.121863
PII dev.121863
PMID 26092846
PMC PMC4510591
MeSH Animal Structures / cytology* Animal Structures / growth & development* Animal Structures / metabolism Animal Structures / ultrastructure Animals Cell Line Cell Polarity Cytoplasm / metabolism Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism Drosophila melanogaster / ultrastructure Dyneins / metabolism Epistasis, Genetic I-kappa B Kinase / metabolism* Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Models, Biological Protein Binding Protein Transport
IF 5.611
引用数 5
WOS 分野 DEVELOPMENTAL BIOLOGY
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